Diagram of the molecular signaling cascades that are involved in myofibrillar muscle protein synthesis and mitochondrial biogenesis in response to physical exercise and specific amino acids or their derivatives (primarily leucine and HMB).
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One week after the initial injection, the Matrigel plug was removed, photographed, weighed, and homogenized in lysis buffer and incubated for 24 hours at 48C and then, disrupted with a Polytron (PRO250, PRO Scientific, Monroe, CT).
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